Rabbit anti-Human DBT Polyclonal Antibody

The antibody against DBT was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.

ADA-08766A

Number

Cat.No	ADA-08766A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	DBT
Target Synonyms	E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCKDH-E2; BCOADC-E2; DBT	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Rat brain, 293T, A375, HepG2, Mouse brain, Mouse lung, Rat liver	Application	ELISA, WB, IF/ICC, IHC-P

Cat.No

ADA-08766A

Clonality

Polyclonal

Host Species

Rabbit

Target Name

DBT

Target Synonyms

E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCKDH-E2; BCOADC-E2; DBT

Form

Liquid

Species Reactivity

Human, Mouse, Rat

Isotype

IgG

Storage Buffer

50% Glycerol, PBS with 0.05% proclin300, pH7.3.

Purification Method

Affinity purification

Positive Samples

Rat brain, 293T, A375, HepG2, Mouse brain, Mouse lung, Rat liver

Application

ELISA, WB, IF/ICC, IHC-P

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3).	Target Species	Human
Uniprot ID	P11182	Immunogen Sequence

Immunogen Description

Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3).

Target Species

Human

Uniprot ID

P11182

Immunogen Sequence

Background Information

Uniprot Id

P11182
Target Species

Human
Target Name

DBT
Target Full Name

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Target Function

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
Target Involvement

Maple syrup urine disease 2 (MSUD2)
Target Subcellular Location

Mitochondrion matrix.
Target Protein Families

2-oxoacid dehydrogenase family
Target Synonyms

DBT; BCATE2; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; mitochondrial; EC 2.3.1.168; 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; Branched chain 2-oxo-acid dehydrogenase complex component E2; BCOADC-E2; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue; 2-methylpropanoyl)transferase
Target Background

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Rabbit anti-Human DBT Polyclonal Antibody

Specifications

Immunogen Information

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