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The antibody against DDX47 was raised in rabbit using the Recombinant Human Probable ATP-dependent RNA helicase DDX47 protein (225-460AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
The antibody against DDX47 was raised in rabbit using the Recombinant Human Probable ATP-dependent RNA helicase DDX47 protein (225-460AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in: HeLa whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate | All lanes: DDX47 antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 51, 46 kDa | Observed band size: 51 kDa
| Cat.No | ADC-01401A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | DDX47 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | IgG | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | >95%, Protein G purified | Conjugate | Non-conjugated |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Recombinant Human Probable ATP-dependent RNA helicase DDX47 protein (225-460AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9H0S4 |
Uniprot Id
Q9H0S4
Target Species
Human
Target Name
DDX47
Target Full Name
Probable ATP-dependent RNA helicase DDX47
Target Function
Involved in apoptosis. May have a role in rRNA processing and mRNA splicing. Associates with pre-rRNA precursors.
Target Subcellular Location
Nucleus, nucleolus. Note=Localizes in the nucleolar-organizing region during ribosome biogenesis.
Target Protein Families
DEAD box helicase family, DDX47/RRP3 subfamily
Target Tissue Specificity
Expressed in skin, lung and breast. Also expressed in the brain.
Target Synonyms
DDX47; DDX47_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 47; DEAD box helicase 47; DEAD box protein 47; DKFZp564O176 ; E4 DBP; FLJ30012 ; HGNC:18682; HQ0256 ; MSTP162 ; Probable ATP dependent RNA helicase DDX47; Probable ATP-dependent RNA helicase DDX47
Target Background
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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