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The antibody against ECHS1 was raised in rabbit using the Recombinant Human Enoyl-CoA hydratase, mitochondrial protein (28-290AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
The antibody against ECHS1 was raised in rabbit using the Recombinant Human Enoyl-CoA hydratase, mitochondrial protein (28-290AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
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Immunohistochemistry of paraffin-embedded human liver tissue using ECHS1 Antibody at dilution of 1:1002
| Cat.No | ADC-16200A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ECHS1 |
| Target Synonyms | ECHS1Enoyl-CoA hydratase antibody; mitochondrial antibody; EC 4.2.1.17 antibody; Enoyl-CoA hydratase 1 antibody; Short-chain enoyl-CoA hydratase antibody; SCEH antibody | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Antigen affinity purified |
| Conjugate | Non-conjugated | Application | ELISA, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human Enoyl-CoA hydratase, mitochondrial protein (28-290AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P30084 |
Uniprot Id
P30084
Target Species
Human
Target Name
ECHS1
Target Full Name
Enoyl-CoA hydratase, mitochondrial
Target Function
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Target Involvement
Mitochondrial short-chain enoyl-CoA hydratase 1 deficiency (ECHS1D)
Target Subcellular Location
Mitochondrion matrix.
Target Protein Families
Enoyl-CoA hydratase/isomerase family
Target Tissue Specificity
Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
Target Research Area
Cancer
Target Synonyms
ECHS1Enoyl-CoA hydratase; mitochondrial; EC 4.2.1.17; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH
Target Background
The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.
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