Mouse anti-Human ERK1 / ERK2 Monoclonal Antibody

The antibody against ERK1 / ERK2 was raised in Mouse using a synthetic peptide of human ERK1 / ERK2 as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-13033A

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Western blot analysis of various lysates, using ERK1 / ERK2 Mouse mAb at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Specifications

Cat.No	ADA-13033A	Clonality	Monoclonal
Host Species	Mouse	Target Name	ERK1 / ERK2
Form	Liquid	Species Reactivity	Human, Mouse, Rat
Isotype	IgG	Storage Buffer	50% Glycerol, PBS with 0.02% sodium azide, pH7.3.
Purification Method	Affinity purification	Positive Samples	C6, HeLa, NIH/3T3, 293T, A-431, PC12, RAW 264.7
Application	ELISA, WB

Immunogen Information

Immunogen Description	A synthetic peptide of human ERK1 / ERK2	Target Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.	Uniprot ID	P28482, P27361

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