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The antibody against GAPDH was raised in mouse using the Recombinant Human GAPDH protein (2-335AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IP, IF.
The antibody against GAPDH was raised in mouse using the Recombinant Human GAPDH protein (2-335AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IP, IF.
$200.00
Western BlotPositive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min
Western BlotPositive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min2
Western BlotPositive WB detected in: Rat heart tissue, Rat liver tissue, Rat spleen tissue, Rat brain tissue, Rat skeletal tissue, Rat stomach tissue, Rat kidney tissue All lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min3
| Cat.No | ADC-57182A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Mouse | Target Name | GAPDH |
| Target Synonyms | GAPDH; G3PD; GAPD; MGC88685 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat, Rabbit | Isotype | IgG1 |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, IF, IHC, WB, IP |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human GAPDH protein (2-335AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P04406 |
Uniprot Id
P04406
Target Species
Human
Target Name
GAPDH
Target Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Target Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Target Subcellular Location
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.
Target Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Target Research Area
Apoptosis, Neuroscience
Target Synonyms
38 kDa BFA-dependent ADP-ribosylation substrate; aging associated gene 9 protein; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; Epididymis secretory sperm binding protein Li 162eP; G3P_HUMAN; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; glyceraldehyde 3-PDH; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS; p38 component; OCT1 coactivator in S phase; 38-KD component; peptidyl cysteine S nitrosylase GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; wu:fb33a10
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