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The antibody against GIF was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 19-417 of human GIF (NP_005133.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against GIF was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 19-417 of human GIF (NP_005133.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of Mouse stomach, using GIF Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
| Cat.No | ADA-08214A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | GIF |
| Target Synonyms | GIF; IF; IFMH; INF; TCN3; gastric intrinsic factor | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse stomach | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 19-417 of human GIF (NP_005133.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P27352 | Immunogen Sequence |
Uniprot Id
P27352
Target Species
Human
Target Name
CBLIF
Target Full Name
Cobalamin binding intrinsic factor
Target Function
Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.
Target Involvement
Hereditary intrinsic factor deficiency (IFD)
Target Subcellular Location
Secreted.
Target Protein Families
Eukaryotic cobalamin transport proteins family
Target Tissue Specificity
Gastric mucosa.
Target Research Area
Immunology
Target Synonyms
CBLIF; GIF; IFMH; Cobalamin binding intrinsic factor; Gastric intrinsic factor; Intrinsic factor; IF; INF
Target Background
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.
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