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The antibody against GRPEL1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
The antibody against GRPEL1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
Western blot analysis of lysates from Mouse liver, using GRPEL1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-03867A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | GRPEL1 |
| Target Synonyms | GrpE; HMGE; mt-GrpE#1; GRPEL1 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse liver | Application | ELISA, WB, IF/ICC, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q9HAV7 | Immunogen Sequence |
Uniprot Id
Q9HAV7
Target Species
Human
Target Name
GRPEL1
Target Full Name
GrpE protein homolog 1, mitochondrial
Target Function
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
Target Subcellular Location
Mitochondrion matrix.
Target Protein Families
GrpE family
Target Research Area
Signal Transduction
Target Synonyms
FLJ25609; GREPEL 1; GREPEL1; GrpE like 1; GrpE like 1 mitochondrial; GrpE like protein cochaperone; GrpE protein homolog 1 mitochondrial; GrpE protein homolog 1; mitochondrial; GRPE1_HUMAN; GRPEL 1; Grpel1; HMGE; Mt GrpE#1; Mt-GrpE#1
Target Background
Enables identical protein binding activity and unfolded protein binding activity. Predicted to be involved in protein import into mitochondrial matrix. Located in mitochondrial matrix and nucleoplasm.
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