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The antibody against HCLS1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 397(G-D-Y(p)-E-E) derived from Human HS1 . as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against HCLS1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 397(G-D-Y(p)-E-E) derived from Human HS1 . as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from JK cells (Lane 2), using HS1 (Phospho-Tyr397) Antibody.The lane on the left is treated with antigen-specific peptide.
| Cat.No | ADC-42670A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | HCLS1 |
| Form | Liquid | Species Reactivity | Human |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of tyrosine 397(G-D-Y(p)-E-E) derived from Human HS1 . | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P14317 |
Uniprot Id
P14317
Target Species
Human
Target Name
HCLS1
Target Full Name
Hematopoietic lineage cell-specific protein
Target Function
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Target Subcellular Location
Membrane; Peripheral membrane protein. Cytoplasm. Mitochondrion.
Target Tissue Specificity
Expressed only in tissues and cells of hematopoietic origin.
Target Synonyms
Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; HS1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75
Target Background
Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and protein kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of protein phosphorylation; and regulation of transcription, DNA-templated. Located in cytosol; nucleus; and plasma membrane. Part of transcription regulator complex.
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