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The antibody against JMJD7 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human JMJD7 (NP_001108104.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against JMJD7 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human JMJD7 (NP_001108104.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of various lysates, using JMJD7 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
| Cat.No | ADA-02524A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | JMJD7 |
| Target Synonyms | JMJD7 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, Rat heart, Daudi, Mouse brain | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human JMJD7 (NP_001108104.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P0C870 | Immunogen Sequence |
Uniprot Id
P0C870
Target Species
Human
Target Name
JMJD7
Target Full Name
Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7
Target Function
Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA).
Target Subcellular Location
Nucleus. Cytoplasm.
Target Research Area
Cell Biology
Target Synonyms
JMJD7; Bifunctional peptidase and; 3S)-lysyl hydroxylase JMJD7; EC 1.14.11.63; EC 3.4.-.-; JmjC domain-containing protein 7; Jumonji domain-containing protein 7; L-lysine; 3S)-hydroxylase JMJD7
Target Background
This gene encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein.
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