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The antibody against KLF5 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against KLF5 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of HeLa, using KLF5 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
| Cat.No | ADA-11855A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | KLF5 |
| Target Synonyms | CKLF; IKLF; BTEB2; KLF5 | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q13887 | Immunogen Sequence |
Uniprot Id
Q13887
Target Species
Human
Target Name
KLF5
Target Full Name
Krueppel-like factor 5
Target Function
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Target Subcellular Location
Nucleus.
Target Protein Families
Krueppel C2H2-type zinc-finger protein family
Target Tissue Specificity
Expressed only in testis and placenta.
Target Synonyms
Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2
Target Background
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants.
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