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The antibody against LIMK2 was raised in rabbit using the Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC, IF.
The antibody against LIMK2 was raised in rabbit using the Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC, IF.
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Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2(Phospho-Thr505) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2(Phospho-Thr505) Antibody(left) or the same antibody preincubated with blocking peptide(right).2
Immunofluorescence staining of methanol-fixed Hela cells using LIMK2(Phospho-Thr505) Antibody.3
| Cat.No | ADC-43742A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | LIMK2 |
| Target Synonyms | LIM domain kinase 2 antibody; LIM kinase 2 antibody; LIMK 2 antibody; LIMK-2 antibody; Limk2 antibody; LIMK2_HUMAN antibody | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) |
| Application | ELISA, IF, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P53671 |
Uniprot Id
P53671
Target Species
Human
Target Name
LIMK2
Target Full Name
LIM domain kinase 2
Target Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1.
Target Subcellular Location
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.; [Isoform LIMK2a]: Cytoplasm. Nucleus.; [Isoform LIMK2b]: Cytoplasm. Cytoplasm, perinuclear region. Nucleus.
Target Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Target Synonyms
LIM domain kinase 2; LIM kinase 2; LIMK 2; LIMK-2; Limk2; LIMK2_HUMAN
Target Background
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
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