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The antibody against MAD1L1 was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAD1L1(NP_003541.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against MAD1L1 was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAD1L1(NP_003541.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of lysates from Rat thymus, using MAD1L1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-08291A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MAD1L1 |
| Target Synonyms | MAD1; MVA7; PIG9; TP53I9; TXBP181; MAD1L1 | Form | Liquid |
| Species Reactivity | Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat thymus | Application | ELISA, WB |
| Immunogen Description | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAD1L1(NP_003541.2). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREV | Uniprot ID | Q9Y6D9 |
Uniprot Id
Q9Y6D9
Target Species
Human
Target Name
MAD1L1
Target Full Name
Mitotic spindle assembly checkpoint protein MAD1
Target Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.; Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.
Target Involvement
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Target Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Nucleus envelope. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 3]: Cytoplasm.
Target Protein Families
MAD1 family
Target Tissue Specificity
[Isoform 1]: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).; [Isoform 3]: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).
Target Synonyms
hMAD1; HsMAD1; MAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181
Target Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
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