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The antibody against MED20 was raised in rabbit using the Recombinant Human Mediator of RNA polymerase II transcription subunit 20 protein (1-212AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF.
The antibody against MED20 was raised in rabbit using the Recombinant Human Mediator of RNA polymerase II transcription subunit 20 protein (1-212AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF.
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Immunohistochemistry of paraffin-embedded human colon cancer using MED20 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using MED20 Antibody at dilution of 1:1002
Immunofluorescent analysis of Hela cells using MED20 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)3
| Cat.No | ADC-15048A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MED20 |
| Target Synonyms | MED20 antibody; TRFP antibody; Mediator of RNA polymerase II transcription subunit 20 antibody; Mediator complex subunit 20 antibody; TRF-proximal protein homolog antibody; hTRFP antibody | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, IF, IHC |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human Mediator of RNA polymerase II transcription subunit 20 protein (1-212AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9H944 |
Uniprot Id
Q9H944
Target Species
Human
Target Name
MED20
Target Full Name
Mediator of RNA polymerase II transcription subunit 20
Target Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Target Subcellular Location
Nucleus.
Target Protein Families
Mediator complex subunit 20 family
Target Synonyms
MED20; TRFP; Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP
Target Background
This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.
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