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The antibody against MEST was raised in rabbit using the Recombinant Human Mesoderm-specific transcript homolog protein (146-335AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC, IF.
The antibody against MEST was raised in rabbit using the Recombinant Human Mesoderm-specific transcript homolog protein (146-335AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC, IF.
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Western blotAll lanes: MEST antibody at 1:500+ Mouse kidney tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39, 38, 34 kDaObserved band size: 39 kDa
Western BlotPositive WB detected in: NTERA-2 whole cell lysate, U87 whole cell lysate, MCF7 whole cell lysate, HT29 whole cell lysate All lanes: MEST antibody at 1:500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39, 38, 34 kDa2
Immunohistochemistry?of?paraffin-embedded?human?Pancreatic tissue?using?MEST Antibody?at?dilution?of?1:503
| Cat.No | ADC-17284A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MEST |
| Target Synonyms | MEST; PEG1; Mesoderm-specific transcript homolog protein; Paternally-expressed gene 1 protein | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Antigen affinity purified |
| Conjugate | Non-conjugated | Application | ELISA, IF, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human Mesoderm-specific transcript homolog protein (146-335AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q5EB52 |
Uniprot Id
Q5EB52
Target Species
Human
Target Name
MEST
Target Full Name
Mesoderm-specific transcript homolog protein
Target Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Target Protein Families
AB hydrolase superfamily
Target Tissue Specificity
Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is exp
Target Synonyms
MEST; PEG1; Mesoderm-specific transcript homolog protein; Paternally-expressed gene 1 protein
Target Background
This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.
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