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The antibody against MOB1A/B was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of human MOB1A/B (NP_060691.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against MOB1A/B was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of human MOB1A/B (NP_060691.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of various lysates, using MOB1A/B Rabbit mAb at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-16434A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MOB1A/B |
| Target Synonyms | MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MABKL1B; MOBKL1B; MOB1A/B | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | C6, Daudi, HuH-7(Low expression control), K-562 | Application | ELISA, WB, IF/ICC |
| Immunogen Description | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MOB1A/B (NP_060691.2). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | MSFLFSSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRQAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCTEASCPVMSAGPRYEYHWADG | Uniprot ID | Q9H8S9, Q7l9L4 |
Uniprot Id
Q9H8S9, Q7l9L4
Target Synonyms
MOB1A/MOB1B
Target Background
The protein MOB1 is considered to be one of the core components of the mammalian Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. MOB1A and MOB1B, which have 95% sequence identity, play redundant biological roles and are both termed MOB1.
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