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The antibody against MYOD1 was raised in rabbit using the Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against MYOD1 was raised in rabbit using the Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD(Phospho-Ser200) Antibody.
| Cat.No | ADC-43787A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MYOD1 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P15172 |
Uniprot Id
P15172
Target Species
Human
Target Name
MYOD1
Target Full Name
Myoblast determination protein 1
Target Function
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Target Subcellular Location
Nucleus.
Target Synonyms
bHLHc1; Class C basic helix-loop-helix protein 1; MYF 3; Myf-3; MYF3; Myoblast determination protein 1; Myod 1; MYOD; MYOD1; MYOD1_HUMAN; Myogenic differentiation 1; Myogenic factor 3; Myogenic factor MYF 3; Myogenin D1; PUM
Target Background
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
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