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The antibody against NPM1 was raised in rabbit using the Peptide sequence around phosphorylation site of Threonine 199(R-D-T(p)-P-A) derived from Human NPM. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against NPM1 was raised in rabbit using the Peptide sequence around phosphorylation site of Threonine 199(R-D-T(p)-P-A) derived from Human NPM. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from Mouse heart tissue using NPM (Phospho-Thr199) Antibody.The lane on the left is treated with the antigen-specific peptide.
| Cat.No | ADC-42991A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | NPM1 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of Threonine 199(R-D-T(p)-P-A) derived from Human NPM. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P06748 |
Uniprot Id
P06748
Target Species
Human
Target Name
NPM1
Target Full Name
Nucleophosmin
Target Function
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes.
Target Involvement
A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
Target Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
Target Protein Families
Nucleoplasmin family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
B23; MGC104254; NO38; NPM; NPM_HUMAN; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; nucleophosmin nucleoplasmin family member 1; Nucleophosmin/nucleoplasmin family member 1; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398
Target Background
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
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