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The antibody against NSUN4 was raised in rabbit using the Recombinant Human 5-methylcytosine rRNA methyltransferase NSUN4 protein (26-284AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC.
The antibody against NSUN4 was raised in rabbit using the Recombinant Human 5-methylcytosine rRNA methyltransferase NSUN4 protein (26-284AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC.
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Western BlotPositive WB detected in: Jurkat whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysateAll lanes: NSUN4 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44, 17, 22, 38 kDaObserved band size: 44 kDa
| Cat.No | ADC-24226A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | NSUN4 |
| Target Synonyms | 5 methylcytosine rRNA methyltransferase NSUN4 antibody; EC=2.1.1.- antibody; NOL1/NOP2/Sun domain family member 4 antibody; NOL1/NOP2/Sun domain family, member 4 antibody; NOP2/Sun domain family, member 4 antibody; Nsun4 antibody; NSUN4_HUMAN antibody; Putative methyltransferase NSUN4 antibody; RP4-603I14.2 antibody; Shtap antibody; Sperm head and tail associated protein antibody | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human 5-methylcytosine rRNA methyltransferase NSUN4 protein (26-284AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q96CB9 |
Uniprot Id
Q96CB9
Target Species
Human
Target Name
NSUN4
Target Full Name
5-cytosine rRNA methyltransferase NSUN4
Target Function
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Target Subcellular Location
Mitochondrion.
Target Protein Families
Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
Target Synonyms
5 methylcytosine rRNA methyltransferase NSUN4; EC=2.1.1.-; NOL1/NOP2/Sun domain family member 4; NOL1/NOP2/Sun domain family, member 4; NOP2/Sun domain family, member 4; Nsun4; NSUN4_HUMAN; Putative methyltransferase NSUN4; RP4-603I14.2; Shtap; Sperm head and tail associated protein
Target Background
Enables rRNA (cytosine-C5-)-methyltransferase activity. Involved in rRNA methylation. Part of mitochondrial large ribosomal subunit.
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