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The antibody against PHF21A was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 461-680 of human PHF21A (NP_001095272.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against PHF21A was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 461-680 of human PHF21A (NP_001095272.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of Mouse liver, using PHF21A Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-03082A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PHF21A |
| Target Synonyms | BHC80; NEDMS; BM-006; IDDBCS; PHF21A | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse liver | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 461-680 of human PHF21A (NP_001095272.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q96BD5 | Immunogen Sequence |
Uniprot Id
Q96BD5
Target Species
Human
Target Name
PHF21A
Target Full Name
PHD finger protein 21A
Target Function
Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
Target Subcellular Location
Nucleus.
Target Tissue Specificity
Highly expressed in brain. Expressed at lower level in other tissues, including heart, kidney, liver, lung and skeletal muscle. Abundantly expressed in fetal brain.
Target Synonyms
BHC80a; BM-006; BRAF35-HDAC complex protein BHC80; BRAF35/HDAC2 complex (80 kDa); KIAA1696; PF21A_HUMAN; PHD finger protein 21A; PHF21A
Target Background
The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).
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