Rabbit anti-Human Phospho-MKK3-S189+MKK6-S207 Monoclonal Antibody

The antibody against Phospho-MKK3-S189+MKK6-S207 was raised in Rabbit using a synthetic phosphorylated peptide around S189/S207 of human MKK3/MKK6 as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.

ADA-15587A

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Western blot analysis of lysates from HeLa cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Specifications

Cat.No	ADA-15587A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	Phospho-MKK3-S189+MKK6-S207
Target Synonyms	MAP2K3/MAP2K6; Phospho-MKK3-S189+MKK6-S207	Form	Liquid
Species Reactivity	Human, Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	HeLa+UV, NIH/3T3+UV	Application	ELISA, WB, IF/ICC

Immunogen Information

Immunogen Description	A synthetic phosphorylated peptide around S189/S207 of human MKK3/MKK6.	Target Species	Human
Immunogen Sequence	VDSVA	Uniprot ID	P46734, P52564

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