Rabbit anti-Human PIP5K1A Polyclonal Antibody

Uniprot Id

Q99755

Target Species

Human

Target Name

PIP5K1A

Target Full Name

Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha

Target Function

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility. PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis. Can also use phosphatidylinositol (PtdIns) as substrate in vitro. Together with PIP5K1C, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps. Promotes particle ingestion by activating the WAS GTPase-binding protein that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, IP3 and DAG, that will mobilize internal calcium and drive keratinocyte differentiation. Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Together with PIP5K1C has a role during embryogenesis. Independently of its catalytic activity, is required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of the small GTPase RAC1 to the plasma membrane. Also functions in the nucleus where it acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.

Target Subcellular Location

Cell membrane. Cytoplasm. Nucleus. Nucleus speckle. Cell projection, ruffle. Cell projection, lamellipodium.

Target Tissue Specificity

Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.

Target Synonyms

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4 phosphate 5 kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type I alpha; Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; PI4P5K Ia; PI51A_HUMAN; PIP5K A; PIP5K1-alpha; Pip5k1a; PIP5KIalpha; PtdIns(4)P-5-kinase 1 alpha

Target Background

Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.