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The antibody against PPAT was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against PPAT was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of Rat spleen, using PPAT Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-08610A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PPAT |
| Target Synonyms | GPAT; PRAT; ATASE; PPAT | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat spleen | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q06203 | Immunogen Sequence |
Uniprot Id
Q06203
Target Species
Human
Target Name
PPAT
Target Full Name
Amidophosphoribosyltransferase
Target Synonyms
GPAT; PRAT; ATASE; PPAT
Target Background
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
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