Rabbit anti-Human PRAME Polyclonal Antibody

The antibody against PRAME was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-10207A

Number

Cat.No	ADA-10207A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	PRAME
Target Synonyms	MAPE; OIP4; CT130; OIP-4; PRAME	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.01% thimerosal, pH7.3.	Purification Method	Affinity purification
Positive Samples	Mouse liver, Mouse lung, Rat lung, U-251MG, U2OS	Application	ELISA, WB

Cat.No

ADA-10207A

Clonality

Polyclonal

Host Species

Rabbit

Target Name

PRAME

Target Synonyms

MAPE; OIP4; CT130; OIP-4; PRAME

Form

Liquid

Species Reactivity

Human, Mouse, Rat

Isotype

IgG

Storage Buffer

50% Glycerol, PBS with 0.01% thimerosal, pH7.3.

Purification Method

Affinity purification

Positive Samples

Mouse liver, Mouse lung, Rat lung, U-251MG, U2OS

Application

ELISA, WB

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1).	Target Species	Human
Immunogen Sequence	MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVK	Uniprot ID	P78395

Immunogen Description

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1).

Target Species

Human

Immunogen Sequence

MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVK

Uniprot ID

P78395

Background Information

Uniprot Id

P78395
Target Species

Human
Target Name

PRAME
Target Full Name

Melanoma antigen preferentially expressed in tumors
Target Function

Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
Target Subcellular Location

Nucleus. Chromosome. Cytoplasm. Golgi apparatus. Cell membrane.
Target Protein Families

PRAME family
Target Tissue Specificity

Expressed in testis. Detected in samples of kidney, brain and skin.
Target Research Area

Apoptosis
Target Synonyms

4930534P07Rik; Cancer/testis antigen 130; CT130; MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4 ; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; PRAME_HUMAN; Preferentially expressed antigen in melanoma ; Preferentially expressed antigen of melanoma; RP23-250F8.3
Target Background

This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Rabbit anti-Human PRAME Polyclonal Antibody

Specifications

Immunogen Information

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