Rabbit anti-Human PUF60 Polyclonal Antibody

Uniprot Id

Q9UHX1

Target Species

Human

Target Name

PUF60

Target Full Name

Poly(U)-binding-splicing factor PUF60

Target Function

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Target Involvement

Verheij syndrome (VRJS)

Target Subcellular Location

Nucleus. Note=Colocalizes partially with TROVE2.

Target Protein Families

RRM half pint family

Target Tissue Specificity

Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, ske

Target Synonyms

60 kDa poly(U)-binding-splicing factor; FBP-interacting repressor; FIR; FLJ31379; FUSE-binding protein-interacting repressor; Poly U binding splicing factor 60KDa; Poly(U) binding splicing factor 60; Poly(U)-binding-splicing factor PUF60; poly-U binding splicing factor PUF60; PUF60; PUF60_HUMAN; pyrimidine tract binding splicing factor ; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; RoBPI; siah binding protein 1; Siah-binding protein 1; Siah-BP1; SIAHBP1

Target Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.