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The antibody against PUS10 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against PUS10 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
| Cat.No | ADA-04713A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PUS10 |
| Target Synonyms | DOBI; Hup10; CCDC139; PUS10 | Form | Liquid |
| Species Reactivity | Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse spleen, Mouse testis, Mouse thymus, Rat thymus | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q3MIT2 | Immunogen Sequence |
Uniprot Id
Q3MIT2
Target Species
Human
Target Name
PUS10
Target Full Name
tRNA pseudouridine synthase Pus10
Target Function
Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
Target Subcellular Location
Nucleus. Cytoplasm. Mitochondrion.
Target Protein Families
Pseudouridine synthase Pus10 family
Target Synonyms
CCDC139; Coiled coil domain containing 139; Coiled coil domain containing protein 139; Coiled-coil domain-containing protein 139; DOBI; FLJ32312; MGC126729; Pseudouridine synthase 10; Pseudouridylate synthase 10; Psi55 synthase; pus10; PUS10_HUMAN; Putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA pseudouridylate synthase; tRNA uridine isomerase; tRNA-uridine isomerase
Target Background
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).
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