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The antibody against PYCR1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against PYCR1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of HepG2, using PYCR1 Rabbit pAb at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
| Cat.No | ADA-11819A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PYCR1 |
| Target Synonyms | P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B; PYCR1 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HepG2 | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P32322 | Immunogen Sequence |
Uniprot Id
P32322
Target Species
Human
Target Name
PYCR1
Target Full Name
Pyrroline-5-carboxylate reductase 1, mitochondrial
Target Function
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Target Involvement
Cutis laxa, autosomal recessive, 2B (ARCL2B); Cutis laxa, autosomal recessive, 3B (ARCL3B)
Target Subcellular Location
Mitochondrion.
Target Protein Families
Pyrroline-5-carboxylate reductase family
Target Synonyms
PYCR1; Pyrroline-5-carboxylate reductase 1; mitochondrial; P5C reductase 1; P5CR 1; EC 1.5.1.2
Target Background
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.
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