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The antibody against PYCR2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
The antibody against PYCR2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.
Western blot analysis of various lysates, using PYCR2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-05239A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PYCR2 |
| Target Synonyms | HLD10; P5CR2; PYCR2 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, A-431, THP-1 | Application | ELISA, WB, IF/ICC, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | MADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD | Uniprot ID | Q96C36 |
Uniprot Id
Q96C36
Target Species
Human
Target Name
PYCR2
Target Full Name
Pyrroline-5-carboxylate reductase 2
Target Function
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Involved in cellular response to oxidative stress.
Target Involvement
Leukodystrophy, hypomyelinating, 10 (HLD10)
Target Subcellular Location
Cytoplasm. Mitochondrion.
Target Protein Families
Pyrroline-5-carboxylate reductase family
Target Tissue Specificity
Detected in erythrocytes (at protein level). Expressed in fetal brain.
Target Synonyms
1810018M05Rik; FLJ54750; Leftb; OTTHUMP00000035614; P5C reductase 2; P5CR 2; P5CR2; P5CR2_HUMAN; PYCR 2; PYCR2; Pyrroline 5 carboxylate reductase 2; Pyrroline 5 carboxylate reductase family member 2; Pyrroline 5 carboxylate reductase isoform; Pyrroline-5-carboxylate reductase 2; RP4-559A3.4
Target Background
This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene.
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