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The antibody against SF-1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 218-461 of human SF-1 (NP_004950.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against SF-1 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 218-461 of human SF-1 (NP_004950.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of Rat brain, using SF-1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-10668A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SF-1 |
| Target Synonyms | NR5A1; AD4BP; ELP; FTZ1; FTZF1; POF7; SF-1; SF1; SPGF8; SRXY3; hSF-1; SRXX4; steroidogenic factor 1 | Form | Liquid |
| Species Reactivity | Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat brain | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 218-461 of human SF-1 (NP_004950.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q13285 | Immunogen Sequence |
Uniprot Id
Q13285
Target Species
Human
Target Name
NR5A1
Target Full Name
Steroidogenic factor 1
Target Function
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Target Involvement
46,XY sex reversal 3 (SRXY3); 46,XX sex reversal 4 (SRXX4); Adrenal insufficiency, NR5A1-related (AINR); Premature ovarian failure 7 (POF7); Spermatogenic failure 8 (SPGF8)
Target Subcellular Location
Nucleus.
Target Protein Families
Nuclear hormone receptor family, NR5 subfamily
Target Tissue Specificity
High expressed in the adrenal cortex, the ovary, the testis, and the spleen.
Target Research Area
Developmental Biology
Target Synonyms
NR5A1; AD4BP; FTZF1; SF1; Steroidogenic factor 1; SF-1; STF-1; hSF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
Target Background
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.
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