Rabbit anti-Human SMURF2 Polyclonal Antibody

The antibody against SMURF2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human SMURF2 (NP_073576.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-10933A

Number

Cat.No	ADA-10933A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	SMURF2
Target Synonyms	SMURF2	Form	Liquid
Species Reactivity	Human	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.02% sodium azide, pH7.3.	Purification Method	Affinity purification
Positive Samples	SW480	Application	ELISA, WB

Cat.No

ADA-10933A

Clonality

Polyclonal

Host Species

Rabbit

Target Name

SMURF2

Target Synonyms

SMURF2

Form

Liquid

Species Reactivity

Human

Isotype

IgG

Storage Buffer

50% Glycerol, PBS with 0.02% sodium azide, pH7.3.

Purification Method

Affinity purification

Positive Samples

SW480

Application

ELISA, WB

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human SMURF2 (NP_073576.1).	Target Species	Human
Immunogen Sequence	DCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTP	Uniprot ID	Q9HAU4

Immunogen Description

Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human SMURF2 (NP_073576.1).

Target Species

Human

Immunogen Sequence

DCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTP

Uniprot ID

Q9HAU4

Background Information

Uniprot Id

Q9HAU4
Target Species

Human
Target Name

SMURF2
Target Full Name

E3 ubiquitin-protein ligase SMURF2
Target Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby downregulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation.; (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding.
Target Subcellular Location

Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
Target Tissue Specificity

Widely expressed.
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

E3 ubiquitin-protein ligase SMURF2; EC 6.3.2.; hSMURF2; MGC138150; Smad specific E3 ubiquitin ligase 2; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMUF2_HUMAN; Smurf2; Ubiquitin protein ligase SMURF2
Target Background

Enables SMAD binding activity; identical protein binding activity; and ubiquitin-protein transferase activity. Involved in negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent SMAD protein catabolic process. Located in nuclear speck. Part of ubiquitin ligase complex.

Rabbit anti-Human SMURF2 Polyclonal Antibody

Specifications

Immunogen Information

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