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The antibody against VISTA / B7-H5 / GI24 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA / B7-H5 / GI24 (NP_071436.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against VISTA / B7-H5 / GI24 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA / B7-H5 / GI24 (NP_071436.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of HLE, using VISTA / B7-H5 / GI24 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-08250A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | VISTA / B7-H5 / GI24 |
| Target Synonyms | VSIR; B7-H5; B7H5; C10orf54; DD1alpha; GI24; PD-1H; PP2135; SISP1; VISTA; V-set immunoregulatory receptor; VISTA / B7-H5 / GI24 | Form | Liquid |
| Species Reactivity | Human, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HLE, Rat spleen | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA / B7-H5 / GI24 (NP_071436.1). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQDSENITAA | Uniprot ID | Q9H7M9 |
Uniprot Id
Q9H7M9
Target Species
Human
Target Name
VSIR
Target Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
Target Function
Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.
Target Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Target Tissue Specificity
Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta
Target Research Area
Cardiovascular
Target Synonyms
B7 H5; B7H5; C10orf54; chromosome 10 open reading frame 54; DD1alpha; GI24; GI24_HUMAN; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; sisp 1; SISP1; stress induced secreted protein 1; UNQ730/PRO1412; V domain Ig suppressor of T cell activation; V set domain containing immunoregulatory receptor; V set immunoregulatory receptor; V type immunoglobulin domain containing suppressor of T cell activation; VISTA
Target Background
Enables endopeptidase activator activity; enzyme binding activity; and identical protein binding activity. Involved in several processes, including negative regulation of cytokine production; positive regulation of macromolecule metabolic process; and regulation of T cell activation. Located in plasma membrane.
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