Rabbit anti-Mouse CD22 Monoclonal Antibody

The antibody against CD22 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22(NP_033975.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.

ADA-13792A

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Western blot analysis of various lysates, using CD22 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Specifications

Cat.No	ADA-13792A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD22
Target Synonyms	Lyb8; Lyb-8; A530093D23; CD22	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	A20	Application	ELISA, WB, IF/ICC

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22(NP_033975.3)	Target Species	Mouse
Immunogen Sequence	SSANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDKATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWMEPIHLNVSEKPF	Uniprot ID	P35329

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