Rabbit anti-Mouse CD272/BTLA Monoclonal Antibody

The antibody against CD272/BTLA was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 30-176 of mouse CD272/BTLA (NP_001032808.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, FC, ELISA.

ADA-15524A

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Western blot analysis of various lysates, using CD272/BTLA Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Specifications

Cat.No	ADA-15524A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD272/BTLA
Target Synonyms	A630002H24; CD272/BTLA	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	A20, Mouse spleen	Application	ELISA, WB, FC

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 30-176 of mouse CD272/BTLA (NP_001032808.2).	Target Species	Mouse
Immunogen Sequence	EKATKRNDEECPVQLTITRNSKQSARTGELFKIQCPVKYCVHRPNVTWCKHNGTICVPLEVSPQLYTSWEENQSVPVFVLHFKPIHLSDNGSYSCSTNFNSQVINSHSVTIHVRERTQNSSEHPLITVSDIPDATNASGPSTMEERP	Uniprot ID	Q7TSA3

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