Rabbit anti-Mouse CD4 Monoclonal Antibody

The antibody against CD4 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 27-394 of mouse CD4 (NP_038516.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IP, FC, ELISA.

ADA-15571A

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Western blot analysis of various lysates, using CD4 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC) :NIH/3T3
Exposure time: 180s.

Specifications

Cat.No	ADA-15571A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD4
Target Synonyms	L3T4; Ly-4; CD4	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.2% BSA, PBS with 0.03% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Mouse thymus	Application	ELISA, WB, FC, IP

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 27-394 of mouse CD4 (NP_038516.1).	Target Species	Mouse
Uniprot ID	P06332	Immunogen Sequence

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