Rabbit anti-Mouse CD44 Monoclonal Antibody

The antibody against CD44 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 23-176 of mouse CD44 (NP_033981.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, FC, ELISA.

ADA-15533A

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Western blot analysis of lysates from C2C12 cells, using CD44 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Specifications

Cat.No	ADA-15533A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD44
Target Synonyms	Ly-24; Pgp-1; HERMES; CD44	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	C2C12	Application	ELISA, WB, FC, IF/ICC

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 23-176 of mouse CD44 (NP_033981.2).	Target Species	Mouse
Immunogen Sequence	QIDLNVTCRYAGVFHVEKNGRYSISRTEAADLCQAFNSTLPTMDQMKLALSKGFETCRYGFIEGNVVIPRIHPNAICAANHTGVYILVTSNTSHYDTYCFNASAPPEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNI	Uniprot ID	P15379

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