Rabbit anti-Mouse CDKN2A/p19ARF Monoclonal Antibody

The antibody against CDKN2A/p19ARF was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse p19ARF (NP_034007.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.

ADA-15554A

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Western blot analysis of various lysates, using p19ARF Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s.

Specifications

Cat.No	ADA-15554A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CDKN2A/p19ARF
Target Synonyms	Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a); CDKN2A/p19ARF	Form	Liquid
Species Reactivity	Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	A20	Application	ELISA, WB, IF/ICC, IHC-P

Immunogen Information

Immunogen Description	A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse p19ARF (NP_034007.1).	Target Species	Mouse
Immunogen Sequence	MGRRFLVTVRIQRAGRPLQERVFLVKFVRSRRPRTASCALAFVNMLLRLERILRRGPHRNPGPGDDDGQRSRSSSSAQLRCRFELRGPHYLLPPGARRSA	Uniprot ID	Q64364-1

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