Rabbit anti-Mouse Cleaved PARP (Asp214) Polyclonal Antibody

The antibody against Cleaved PARP (Asp214) was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 201-300 of Mouse PARP (Asp214) (NP_031441.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-10654A

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Western blot analysis of lysates from NIH/3T3 cells, using Cleaved PARP Rabbit pAb at 1:400 dilution.NIH/3T3 cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Specifications

Cat.No	ADA-10654A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	Cleaved PARP (Asp214)
Target Synonyms	PARP; PPOL; ARTD1; Adprp; Adprt1; msPARP; parp-1; sPARP-1; 5830444G22Rik; Cleaved PARP (Asp214)	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	NIH/3T3+Staurosporine	Application	ELISA, WB

Immunogen Information

Immunogen Description	A synthetic peptide corresponding to a sequence within amino acids 201-300 of Mouse PARP (Asp214) (NP_031441.2).	Target Species	Mouse
Immunogen Sequence	AIKNEGKRKGDEVDGTDEVAKKKSKKGKDKDSSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAILDRVADGMAFGALLPCKECS	Uniprot ID	Q921K2

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