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The antibody against IL12B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of mouse IL12B(NP_001290173.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, FC, ELISA.
The antibody against IL12B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of mouse IL12B(NP_001290173.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, FC, ELISA.
Western blot analysis of various lysates, using IL12B Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-15468A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | IL12B |
| Target Synonyms | p40; Il-12b; Il12p40; Il-12p40; IL12B | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | 293T transfected with IL12B (Mouse) | Application | ELISA, WB, FC, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of mouse IL12B(NP_001290173.1). | Target Species | Mouse |
|---|---|---|---|
| Uniprot ID | P43432 | Immunogen Sequence |
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