Rabbit anti-Mouse IL2 Monoclonal Antibody

The antibody against IL2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 21-169 of mouse IL2 (NP_032392.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-14811A

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Western blot analysis of recombinant mouse IL-2 protein, using IL2 Rabbit mAb at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.2s.

Specifications

Cat.No	ADA-14811A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	IL2
Target Synonyms	Il-2; IL2	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Recombinant Mouse IL-2 protein	Application	ELISA, WB

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 21-169 of mouse IL2 (NP_032392.1).	Target Species	Mouse
Immunogen Sequence	APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ	Uniprot ID	P04351

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