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The antibody against ogt was raised in rabbit using the Recombinant Escherichia coli (strain K12) ogt protein (1-171aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
The antibody against ogt was raised in rabbit using the Recombinant Escherichia coli (strain K12) ogt protein (1-171aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in: recombinant protein | All lanes: ogt Antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 25 kDa | Observed band size: 25 kDa
| Cat.No | ADC-55340A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | OGT |
| Target Synonyms | Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase) ogt b1335 JW1329 | Form | Liquid |
| Species Reactivity | Escherichia coli (strain K12) | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Affinity-chromatography purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Escherichia coli (strain K12) ogt protein (1-171aa) | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P0AFH0 |
Uniprot Id
P0AFH0
Target Species
E.coli
Target Name
OGT
Target Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Target Subcellular Location
Cytoplasm.
Target Protein Families
MGMT family
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