Rabbit anti-Rat Icam1 Polyclonal Antibody

The antibody against Icam1 was raised in Rabbit using the recombinant protein of rat Icam1 as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.

ADA-08138A

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Western blot analysis of various lysates, using Icam1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Specifications

Cat.No	ADA-08138A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	ICAM1
Target Synonyms	CD54; ICAM; Icam1	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Mouse lung, Mouse spleen, Rat lung, Rat spleen	Application	ELISA, WB, IF/ICC

Immunogen Information

Immunogen Description	Recombinant protein of rat Icam1.	Target Species	Rat
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.	Uniprot ID	Q00238

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