Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The gene coding for the Human ASMT protein (1-345aa) is introduced into a plasmid vector, creating recombinant plasmid that is subsequently transformed into e.coli cells. e.coli cells capable of surviving in the presence of a specific antibiotic are selected and then cultured under conditions favorable for the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Post-expression, the recombinant Human ASMT protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human ASMT protein, revealing a purity exceeding 85%.
The gene coding for the Human ASMT protein (1-345aa) is introduced into a plasmid vector, creating recombinant plasmid that is subsequently transformed into e.coli cells. e.coli cells capable of surviving in the presence of a specific antibiotic are selected and then cultured under conditions favorable for the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Post-expression, the recombinant Human ASMT protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human ASMT protein, revealing a purity exceeding 85%.
| Cat.No | ACP04996 | Target Name | ASMT |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-345aa | Mol Weight | 45.9 kDa |
| Protein Length | Full length | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P46597 |
|---|
Uniprot Id
P46597
Target Species
Human
Target Name
ASMT
Target Full Name
Acetylserotonin O-methyltransferase
Target Function
Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity.
Target Protein Families
Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family
Target Tissue Specificity
Expressed in the pineal gland (at protein level). In the retina, very low expression is found at the mRNA level, and not at the protein level.
Target Research Area
Cell Biology
Target Synonyms
acetylserotonin methyltransferase (Y chromosome); acetylserotonin N-methyltransferase; Acetylserotonin O-methyltransferase; ASMT; ASMTY; HIOM_HUMAN; HIOMT; HIOMTY; Hydroxyindole O-methyltransferase; OTTHUMP00000022834; OTTHUMP00000022835; OTTHUMP00000022836
Target Background
This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
