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Insertion of the gene encoding the Human ACTL8 protein (1-366aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Following expression, the recombinant Human ACTL8 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ACTL8 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.
Insertion of the gene encoding the Human ACTL8 protein (1-366aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Following expression, the recombinant Human ACTL8 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ACTL8 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.
| Cat.No | ACP03156 | Target Name | ACTL8 |
|---|---|---|---|
| Target Synonyms | Actin like 8; Actin like protein 8; Actin like protein; Actin-like protein 8; ACTL8; ACTL8_HUMAN; Cancer/testis antigen 57; CT57 | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 1-366aa |
| Mol Weight | 45.4 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9H568 |
|---|
Uniprot Id
Q9H568
Target Species
Human
Target Name
ACTL8
Target Full Name
Actin-like protein 8
Target Subcellular Location
Cytoplasm, cytoskeleton.
Target Protein Families
Actin family
Target Tissue Specificity
Strongly expressed in testis and pancreas. Weak expression in placenta.
Target Research Area
Signal Transduction
Target Synonyms
Actin like 8; Actin like protein 8; Actin like protein; Actin-like protein 8; ACTL8; ACTL8_HUMAN; Cancer/testis antigen 57; CT57
Target Background
Involved in epithelial cell differentiation. Predicted to be located in cytoplasm. Predicted to be part of dynactin complex.
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