Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The DNA coding sequence translated into the human GPT protein sequence (2-496aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human GPT protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 55 kDa protein band was observed on the gel.GPT is a gene providing instructions for making a protein named alanine aminotransferase 1 (abbreviated GPT) in human and belongs to class-I pyridoxal-phosphate-dependent aminotransferase family or alanine aminotransferase subfamily. This protein is mainly expressed in liver, kidney, heart, and skeletal muscles, and expressed at moderate levels in the adipose tissue. This protein is an enzyme catalyzing the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It is involved in cellular nitrogen metabolism and also in in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
The DNA coding sequence translated into the human GPT protein sequence (2-496aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human GPT protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 55 kDa protein band was observed on the gel.GPT is a gene providing instructions for making a protein named alanine aminotransferase 1 (abbreviated GPT) in human and belongs to class-I pyridoxal-phosphate-dependent aminotransferase family or alanine aminotransferase subfamily. This protein is mainly expressed in liver, kidney, heart, and skeletal muscles, and expressed at moderate levels in the adipose tissue. This protein is an enzyme catalyzing the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It is involved in cellular nitrogen metabolism and also in in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
| Cat.No | ACP04778 | Target Name | GPT |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-496aa | Mol Weight | 58.5kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P24298 |
|---|
Uniprot Id
P24298
Target Species
Human
Target Name
GPT
Target Full Name
Alanine aminotransferase 1
Target Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Target Subcellular Location
Cytoplasm.
Target Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Target Tissue Specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Target Research Area
Signal Transduction, Metabolism
Target Synonyms
AAT1; Alanine aminotransferase 1; Alanine aminotransferase; ALAT1_HUMAN; ALT1; Glutamate pyruvate transaminase 1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase (alanine aminotransferase); Glutamic pyruvic transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT 1; gpt; GPT1
Target Background
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
