Recombinant Human ATPase ASNA1 (ASNA1)

Uniprot Id

O43681

Target Species

Human

Target Name

GET3

Target Full Name

ATPase GET3

Target Function

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.

Target Subcellular Location

Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.

Target Protein Families

ArsA ATPase family

Target Tissue Specificity

Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.

Target Research Area

Transport

Target Synonyms

ARSA 1; ARSA; ArsA arsenite transporter ATP binding homolog 1; ArsA arsenite transporter; ATP binding; E. coli; homolog of; 1; ArsA arsenite transporter; ATP-binding; homolog 1 (bacterial); ARSA I; ARSA-I; ARSA1; ARSAI; Arsenical pump driving ATPase; Arsenical pump-driving ATPase; Arsenical resistance ATPase; Arsenite translocating ATPase; Arsenite transporting ATPase; Arsenite-stimulated ATPase; ASNA 1; ASNA I; ASNA-I; ASNA_HUMAN; ASNA1; Asna1 protein ; ASNAI; ATPase ASNA1; GET3; Golgi to ER traffic 3 homolog; hARSA-I; hASNA-I; MGC3821; Transmembrane domain recognition complex 40 kDa ATPase subunit; Transmembrane domain recognition complex; 40kDa; TRC40

Target Background

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion.