Recombinant Human Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), Truncated

The gene encoding the Human HSPG2 protein (4197-4391aa) is inserted into a plasmid vector to form the recombinant plasmid, which is introduced into baculovirus cells. baculovirus cells that have successfully taken up the recombinant plasmid can be selected based on their ability to survive in the presence of a specific antibiotic. The recombinant plasmid-containing baculovirus cells are cultured under conditions that promote the expression of the interested gene. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. After expression, the recombinant Human HSPG2 protein is isolated and purified from the cell lysate through affinity purification. The resulting recombinant Human HSPG2 protein is resolved by denaturing SDS-PAGE, allowing its purity to be estimated, greater than 85%.

ACP04972

Number

Cat.No	ACP04972	Target Name	HSPG2
Form	Liquid or Lyophilized powder	Expression System	Baculovirus
Expression Range	4197-4391aa	Mol Weight	24.4
Protein Length	Partial	Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04972

Target Name

HSPG2

Form

Liquid or Lyophilized powder

Expression System

Baculovirus

Expression Range

4197-4391aa

Mol Weight

24.4

Protein Length

Partial

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P98160

Background Information

Uniprot Id

P98160
Target Species

Human
Target Name

HSPG2
Target Full Name

Basement membrane-specific heparan sulfate proteoglycan core protein
Target Function

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.; Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.; The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
Target Involvement

Schwartz-Jampel syndrome (SJS1); Dyssegmental dysplasia Silverman-Handmaker type (DDSH)
Target Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.
Target Tissue Specificity

Found in the basement membranes.
Target Research Area

Cancer
Target Synonyms

Basement membrane specific heparan sulfate proteoglycan core protein; Endorepellin (domain V region); Heparan sulfate proteoglycan of basement membrane; HSPG 2; HSPG; Hspg2; LG3 peptide; Perlecan; PGBM_HUMAN; PLC; Schwartz Jampel syndrome 1 (chondrodystrophic myotonia); SJA; SJS; SJS1

Recombinant Human Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), Truncated

Specifications

Immunogen Information

Inquire Recombinant Human Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), Truncated Now

Related Products