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The process of expressing the recombinant human BDNF protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 129-247aa of the human BDNF protein and N-terminal 6xHis-SUMO tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-SUMO-tagged recombinant BDNF protein was obtained. This recombinant BDNF protein is characterized by high purity (>90%, SDS-PAGE). This BDNF protein ran along the gel to the band of approximately 30 kDa molecular weight.BDNF is a gene providing instruction of making a protein named brain-derived neurotrophic factor (also abbreviated as BDNF) in human and belongs to NGF-beta family. BDNF has a wide array of functions within the brain, involving supporting differentiation, maturation, and survival of neurons in the nervous system. It is highly abundant in the CNS, gut and other tissues. The levels of BDNF are decreased in many neurodegenerative diseases such as Parkinson's disease (PD), multiple sclerosis (MS) and Huntington's disease.
The process of expressing the recombinant human BDNF protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 129-247aa of the human BDNF protein and N-terminal 6xHis-SUMO tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-SUMO-tagged recombinant BDNF protein was obtained. This recombinant BDNF protein is characterized by high purity (>90%, SDS-PAGE). This BDNF protein ran along the gel to the band of approximately 30 kDa molecular weight.BDNF is a gene providing instruction of making a protein named brain-derived neurotrophic factor (also abbreviated as BDNF) in human and belongs to NGF-beta family. BDNF has a wide array of functions within the brain, involving supporting differentiation, maturation, and survival of neurons in the nervous system. It is highly abundant in the CNS, gut and other tissues. The levels of BDNF are decreased in many neurodegenerative diseases such as Parkinson’s disease (PD), multiple sclerosis (MS) and Huntington’s disease.
| Cat.No | ACP03109 | Target Name | BDNF |
|---|---|---|---|
| Target Synonyms | Abrineurin; ANON2; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 129-247aa |
| Mol Weight | 29.5kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P23560 |
|---|
Uniprot Id
P23560
Target Species
Human
Target Name
BDNF
Target Full Name
Neurotrophic factor BDNF precursor form
Target Function
Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
Target Involvement
Bulimia nervosa 2 (BULN2); Congenital central hypoventilation syndrome (CCHS)
Target Subcellular Location
Secreted.; [BDNF precursor form]: Secreted.
Target Protein Families
NGF-beta family
Target Tissue Specificity
Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Target Research Area
Neuroscience
Target Synonyms
Abrineurin; ANON2; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
Target Background
This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
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