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The gene encoding the Human BTN3A1 protein (30-254aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human BTN3A1 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human BTN3A1 protein, revealing a purity level exceeding 90%.
The gene encoding the Human BTN3A1 protein (30-254aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human BTN3A1 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human BTN3A1 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP03097 | Target Name | BTN3A1 |
|---|---|---|---|
| Target Synonyms | BT3A1_HUMAN; BTN3A1; Butyrophilin subfamily 3 member A1; CD277 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 30-254aa |
| Mol Weight | 40.2kDa | Protein Length | Extracellular Domain |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O00481 |
|---|
Uniprot Id
O00481
Target Species
Human
Target Name
BTN3A1
Target Full Name
Butyrophilin subfamily 3 member A1
Target Function
Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
Target Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Target Protein Families
Immunoglobulin superfamily, BTN/MOG family
Target Tissue Specificity
Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.
Target Research Area
Immunology
Target Synonyms
BT3A1_HUMAN; BTN3A1; Butyrophilin subfamily 3 member A1; CD277
Target Background
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).
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