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The production of this Recombinant Human cry1Ac protein began at the genetic level, where the coding sequence for the cry1Ac protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired CA12 protein. And we finally got the recombinant CA12 protein with the purity of 90%+ determined by SDS-PAGE. The carbonic anhydrase 12 (CA12) gene encodes a zinc metalloenzyme that catalyzes reversible carbon dioxide hydration and thereby modulates a range of biological processes. CA12 is a type I membrane protein that has been found to be expressed in renal cell carcinoma and a range of other cancer types. There is some evidence that CA12 can promote tumor growth by driving micro environmental acidification. And some studies have primarily found that CA12 expression is restricted to certain cell types with maximal expression in terminally differentiated cells. In breast cancer patients, intratumoral CA12 upregulation is associated with better patient prognosis, whereas in oral cancer this gene is associated with tumor invasion and metastasis. Currently, CA12 is known to play central roles in regulating many cancers, but its function in the context of different cancers remains to be fully discussed.
The production of this Recombinant Human cry1Ac protein began at the genetic level, where the coding sequence for the cry1Ac protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired CA12 protein. And we finally got the recombinant CA12 protein with the purity of 90%+ determined by SDS-PAGE. The carbonic anhydrase 12 (CA12) gene encodes a zinc metalloenzyme that catalyzes reversible carbon dioxide hydration and thereby modulates a range of biological processes. CA12 is a type I membrane protein that has been found to be expressed in renal cell carcinoma and a range of other cancer types. There is some evidence that CA12 can promote tumor growth by driving micro environmental acidification. And some studies have primarily found that CA12 expression is restricted to certain cell types with maximal expression in terminally differentiated cells. In breast cancer patients, intratumoral CA12 upregulation is associated with better patient prognosis, whereas in oral cancer this gene is associated with tumor invasion and metastasis. Currently, CA12 is known to play central roles in regulating many cancers, but its function in the context of different cancers remains to be fully discussed.
| Cat.No | ACP02462 | Target Name | CA12 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 25-301aa | Mol Weight | 33.1kDa |
| Protein Length | Extracellular Domain | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O43570 |
|---|
Uniprot Id
O43570
Target Species
Human
Target Name
CA12
Target Full Name
Carbonic anhydrase 12
Target Function
Reversible hydration of carbon dioxide.
Target Involvement
Hyperchlorhidrosis, isolated (HCHLH)
Target Subcellular Location
Membrane; Single-pass type I membrane protein. Cell membrane.
Target Protein Families
Alpha-carbonic anhydrase family
Target Tissue Specificity
Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat gl
Target Research Area
Cancer
Target Synonyms
CA 12; CA XII; CA-XII; CA12; CAH12_HUMAN; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; Carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM RCC 3.1.3; Tumor antigen HOM-RCC-3.1.3
Target Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.
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