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The production of this Recombinant Human CA9 protein started with the CA9 gene synthesis. And then using recombinant DNA technology, the CA9 gene was inserted into an expression vector so that we could get the recombinant express plasmid of CA9. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human CA9 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 85%+ determined by SDS-PAGE.CA9 (also called G250 or MN) is a gene providing an instruction of making a protein named carbonic anhydrase 9 (CA9) in human. The protein encoded by this gene has several alternative names, including carbonate dehydratase IX, carbonic anhydrase IX (CA-IX or CAIX), membrane antigen MN, P54/58N, RCC-associated antigen G250 and pMW1. CAP protein is a transmembrane dimeric metalloenzyme with an extracellular active site that facilitates acid secretion in the gastrointestinal tract. This protein is overexpressed in many types of cancer.
The production of this Recombinant Human CA9 protein started with the CA9 gene synthesis. And then using recombinant DNA technology, the CA9 gene was inserted into an expression vector so that we could get the recombinant express plasmid of CA9. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human CA9 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 85%+ determined by SDS-PAGE.CA9 (also called G250 or MN) is a gene providing an instruction of making a protein named carbonic anhydrase 9 (CA9) in human. The protein encoded by this gene has several alternative names, including carbonate dehydratase IX, carbonic anhydrase IX (CA-IX or CAIX), membrane antigen MN, P54/58N, RCC-associated antigen G250 and pMW1. CAP protein is a transmembrane dimeric metalloenzyme with an extracellular active site that facilitates acid secretion in the gastrointestinal tract. This protein is overexpressed in many types of cancer.
| Cat.No | ACP05089 | Target Name | CA9 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Protein Length | Partial | Purity | >85% (SDS-PAGE) |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q16790 |
|---|
Uniprot Id
Q16790
Target Species
Human
Target Name
CA9
Target Full Name
Carbonic anhydrase 9
Target Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Target Subcellular Location
Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Target Protein Families
Alpha-carbonic anhydrase family
Target Tissue Specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Target Research Area
Metabolism
Target Synonyms
CA-IX; CA9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma-associated antigen G250
Target Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
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