Recombinant Human Cellular nucleic acid-binding protein (CNBP)

Incorporation of the gene encoding the Human CNBP protein (2-170aa) into a plasmid vector forms recombinant plasmid, which is then transformed into e.coli cells. baculovirus cells containing the recombinant plasmid that can survive in the presence of a specific antibiotic are selected and cultured under conditions conducive to the expression of the gene of interest. The protein features a N-terminal 6xHis-SUMO tag fusion. Following expression, the recombinant Human CNBP protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant protein, demonstrating a purity greater than 90%.

ACP04353

Number

Cat.No	ACP04353	Target Name	CNBP
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	2-170aa	Mol Weight	34.6kDa
Protein Length	Full?Length?of?Mature?Protein of Isoform 2	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04353

Target Name

CNBP

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

2-170aa

Mol Weight

34.6kDa

Protein Length

Full?Length?of?Mature?Protein of Isoform 2

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P62633

Background Information

Uniprot Id

P62633
Target Species

Human
Target Name

CNBP
Target Full Name

CCHC-type zinc finger nucleic acid binding protein
Target Function

Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression. Has a role as transactivator of the Myc promoter. Binds single-stranded RNA in a sequence-specific manner.; Binds G-rich elements in target mRNA coding sequences. Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs.; Binds to RNA.; Binds to RNA.; Binds to RNA.; Binds to RNA.; Binds to RNA.
Target Involvement

Dystrophia myotonica 2 (DM2)
Target Subcellular Location

Nucleus. Cytoplasm. Endoplasmic reticulum.; [Isoform 1]: Cytoplasm.; [Isoform 2]: Cytoplasm.; [Isoform 4]: Cytoplasm.; [Isoform 5]: Cytoplasm.; [Isoform 6]: Cytoplasm.; [Isoform 8]: Cytoplasm.
Target Tissue Specificity

Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

CCHC type zinc finger nucleic acid binding protein; Cellular nucleic acid binding protein; Cellular nucleic acid-binding protein; CNBP; CNBP_HUMAN; CNBP1; DM2; Erythroid differentiation related; PROMM; Proximal myotonic myopathy nucleic acid binding protein; RNF163; Sterol regulatory element binding protein; ZCCHC22; Zinc finger protein 273; Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein); Zinc finger protein 9; ZNF9
Target Background

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Recombinant Human Cellular nucleic acid-binding protein (CNBP)

Specifications

Immunogen Information

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